Liquid chromatography coupled with diode array detector as a tool for separation, detection and characterization of secondary metabolites - naphtoquinones in plant tissue samples

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F05%3A00007475" target="_blank" >RIV/62156489:43210/05:00007475 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Liquid chromatography coupled with diode array detector as a tool for separation, detection and characterization of secondary metabolites - naphtoquinones in plant tissue samples

Original language description

Naphthoquinones, compounds of natural origin, mostly appeared as chromatic pigments. They are deposited in cells vacuoles, where they are dissolved (in glycoside form). Naphthoquinones are very toxic, antimicrobial, antifungal, antiviral and antiparasitic effects were observed. The aim of our work was to optimize the high performance liquid chromatography coupled with diode array detector for the determination of naphtoquinones (1,4-naphtoquinone, lawsone; juglone and plumbagin). Detection limits (3 S/N) of naphtoquinones were -- lawson 65 ng.ml-1, 1,4-naftochinon 50 ng.ml-1, juglon 75 ng.ml-1 and plumbagin 39 ng.ml-1. The optimized method was consequently used for the determination of the mentioned naphtoquinones in plant sample (Dionaea muscipula).

Czech name

Kapalinová chromatografie ve spojení s DAD detektorem jako nástroj pro separaci a charakterizaci sekundárních metabolitů - naftochinonů v rostlinných pletivech

Czech description

Naphthoquinones, compounds of natural origin, mostly appeared as chromatic pigments. They are deposited in cells vacuoles, where they are dissolved (in glycoside form). Naphthoquinones are very toxic, antimicrobial, antifungal, antiviral and antiparasitic effects were observed. The aim of our work was to optimize the high performance liquid chromatography coupled with diode array detector for the determination of naphtoquinones (1,4-naphtoquinone, lawsone; juglone and plumbagin). Detection limits (3 S/N) of naphtoquinones were -- lawson 65 ng.ml-1, 1,4-naftochinon 50 ng.ml-1, juglon 75 ng.ml-1 and plumbagin 39 ng.ml-1. The optimized method was consequently used for the determination of the mentioned naphtoquinones in plant sample (Dionaea muscipula).

Type

D - Article in proceedings

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GP525%2F04%2FP132" target="_blank" >GP525/04/P132: Study of protection mechanisms for stress-induced by heavy metals</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Vitamins 2005 - Targeted Nutritional Therapy

ISBN

80-7194-748-2

ISSN

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e-ISSN

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Number of pages

3

Pages from-to

172-174

Publisher name

Univerzita Pardubice

Place of publication

Pardubice, Czech Republic

Event location

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Event date

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Type of event by nationality

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UT code for WoS article

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