An influence of cisplatin on tobacco cell culture Nicotiana tabacum BY-2

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F05%3A00086832" target="_blank" >RIV/62156489:43210/05:00086832 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

VLIV CISPLATINY NA BUNĚČNOU SUSPENZNÍ KULTURU TABÁKU NICOTIANA TABACUM BY-2

Original language description

Derivatives of platinous and platinic complex with strong alkylating effect have important role in treatment of malignant disorders. Cisplatin was introduced to the therapy of these disorders in 1965 followed by carboplatin and oxaliplatin. The spectrumof their cytostatic effects is wide and influences carcinomas of testicles, pulmonary, breast, prostate and of many others. The mechanism of cisplatin effect has been well examined in in vitro models of malignant cell lines, but has never been studied onplant cell lines. The main aim of our work was to study cytotoxic effect of cisplatin on plant cell suspension culture of Nicotiana tabacum BY-2. We observed changing of their viability with increasing cisplatin concentration in culture medium using fluorescent microscopy. Mitotic index was studied too. We also measured morphological structure of nuclei (nucleolus, heterochromatin and euchromatin) as well as mitosis and single phases of mitosis.

Czech name

VLIV CISPLATINY NA BUNĚČNOU SUSPENZNÍ KULTURU TABÁKU NICOTIANA TABACUM BY-2

Czech description

Derivatives of platinous and platinic complex with strong alkylating effect have important role in treatment of malignant disorders. Cisplatin was introduced to the therapy of these disorders in 1965 followed by carboplatin and oxaliplatin. The spectrumof their cytostatic effects is wide and influences carcinomas of testicles, pulmonary, breast, prostate and of many others. The mechanism of cisplatin effect has been well examined in in vitro models of malignant cell lines, but has never been studied onplant cell lines. The main aim of our work was to study cytotoxic effect of cisplatin on plant cell suspension culture of Nicotiana tabacum BY-2. We observed changing of their viability with increasing cisplatin concentration in culture medium using fluorescent microscopy. Mitotic index was studied too. We also measured morphological structure of nuclei (nucleolus, heterochromatin and euchromatin) as well as mitosis and single phases of mitosis.

Type

D - Article in proceedings

CEP classification

ED - Physiology

OECD FORD branch

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Project

<a href="/en/project/GP525%2F04%2FP132" target="_blank" >GP525/04/P132: Study of protection mechanisms for stress-induced by heavy metals</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

MendelNet´05 Agro

ISBN

80-7157-905-X

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

97-97

Publisher name

Mendelova zemědělská a lesnická univerzita v Brně

Place of publication

Brno

Event location

MZLU Brno

Event date

Nov 29, 2005

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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