The Transformation of Pea (Pisum sativum L.) : Applicable Methods of Agrobacterium tumefaciens-mediated Gene Transfer

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F07%3A00094903" target="_blank" >RIV/62156489:43210/07:00094903 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The Transformation of Pea (Pisum sativum L.) : Applicable Methods of Agrobacterium tumefaciens-mediated Gene Transfer

Original language description

Three methods of transformation of pea (Pisum sativum ssp. sativum L. var. medullare) were tested. The most efficient Agrobacterium tumefaciens-mediated T-DNA transfer was obtained using embryonic segments from mature pea seeds as initial explants. The transformation procedure was based on the transfer of the T-DNA region with the reporter gene uidA and selection gene bar. The expression of beta-glucuronidase (GUS) in the regenerated shoots was tested using the histochemical method and the shoots were selected on a medium containing phosphinothricin (PPT). The shoots of putative transformants were rooted and transferred to non-sterile conditions. Transient expression of the uidA gene in the tissues after co-cultivation and in the course of short-term shoot cultivation (confirmed by histochemical analysis of GUS and by RT-PCR of mRNA) was achieved; however, we have not yet succeeded in proving stable incorporation of the transgene in the analysed plants.

Czech name

Transformace hrachu (Pisum sativum L.): Použitelné metody přenosu genů pomocí Agrobacterium tumefaciens

Czech description

Byly testovány tři metody transformace hrachu (Pisum sativum ssp. sativum L. var. medullare).

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/QF3072" target="_blank" >QF3072: Development of transgenic pea (Pisum sativum L.) lines with enhanced resistance to viral pathogens.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Physiologiae Plantarum

ISSN

0137-5881

e-ISSN

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Volume of the periodical

29

Issue of the periodical within the volume

1

Country of publishing house

PL - POLAND

Number of pages

7

Pages from-to

157-163

UT code for WoS article

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EID of the result in the Scopus database

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