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Zeptomole detection of streptavidin using carbon paste electrode and square wave voltammetry

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F07%3A00107904" target="_blank" >RIV/62156489:43210/07:00107904 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14310/07:00050796

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Zeptomole detection of streptavidin using carbon paste electrode and square wave voltammetry

  • Original language description

    We compared the suitability of avidin and streptavidin for avidin-biotin technology in view of the sensitivity of the analysis using square-wave voltammetry. We found out during our preliminary experiments that streptavidin gave approximately 100 times higher electrochemical response in comparison with avidin at the same experimental conditions and concentration. Thus, we used two approaches for streptavidin determination -- analysis directly in electrochemical cell and analysis by adsorptive transfer technique (AdTS). Ten minutes long accumulation on carbon paste electrode surface was ascertained as optimal in both cases. Limits of detection were 0.3 aM (electrochemical cell) and 30 aM (AdTS).

  • Czech name

    Zeptomolární detekce streptavidinu s použitím uhlíkové pastové elektrody a square wave voltametrie

  • Czech description

    V této práci jsme porovnávali využitelnost avidinu a streptavidinu pro avidin-biotinovou technologii z pohledu senzitivity analýzy užitím square-wave voltametrie. Zjistili jsme, že streptavidin poskytuje přibližně 100 krát vyšší elektrochemickou odezvu vporovnáíní s avidinem při stejných experimentálních podmínkách a koncentraci. Pak jsme užili dva přístupy pro stanovení streptavidinu. Přímou analýzu v elektrochemické cele a analýzu pomocí adsorptivní přenosové techniky (AdTS). Desetiminutová akumulacina uhlíkovou pastovou elektrodu byla optimální v obou případech. Limity detekce byly 0.3 aM (elektrochemická cela) a 30 aM (AdTS)

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CG - Electrochemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2007

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Electroanalysis

  • ISSN

    1040-0397

  • e-ISSN

  • Volume of the periodical

    19

  • Issue of the periodical within the volume

    11

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    6

  • Pages from-to

    1177-1182

  • UT code for WoS article

  • EID of the result in the Scopus database