Comparison of prenatal muscle tissue expression profiles of genes CDK4 and TP53BP1 in two pig breeds

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F07%3A00107957" target="_blank" >RIV/62156489:43210/07:00107957 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Comparison of prenatal muscle tissue expression profiles of genes CDK4 and TP53BP1 in two pig breeds

Original language description

This research was focused on investigation of gene expression of selected genes via the real-time quantitative PCR. Specific primers for CDK4 and TP53BP1 were designed on the basis of the sequences clones obtained from the subtractive hybridization and gene expression was analysed. Two groups of samples isolated skeletal muscle-specific RNA were compared, the first one was obtained from the Pietrain adult sows and their fetuses, the second one was obtained from Large White, respectively. The stability values of candidate reference genes (ACTB, EEF1A1, EEF2, GAPDH, H3F, HPRT1, PPIA and TOP2B) were made for both breeds. The expression levels of the investigated genes were varying by adult sows used as control samples. The higher or lower expression levelwas found out in few samples; one of the samples of gene CDK4 was expressed near to 0.001-fold, and two of samples of gene TP53BP1 were expressed higher then 18-fold, 52-fold respectively. The long-range goal of this research is to provi

Czech name

Comparison of prenatal muscle tissue expression profiles of genes CDK4 and TP53BP1 in two pig breeds

Czech description

This research was focused on investigation of gene expression of selected genes via the real-time quantitative PCR. Specific primers for CDK4 and TP53BP1 were designed on the basis of the sequences clones obtained from the subtractive hybridization and gene expression was analysed. Two groups of samples isolated skeletal muscle-specific RNA were compared, the first one was obtained from the Pietrain adult sows and their fetuses, the second one was obtained from Large White, respectively. The stability values of candidate reference genes (ACTB, EEF1A1, EEF2, GAPDH, H3F, HPRT1, PPIA and TOP2B) were made for both breeds. The expression levels of the investigated genes were varying by adult sows used as control samples. The higher or lower expression levelwas found out in few samples; one of the samples of gene CDK4 was expressed near to 0.001-fold, and two of samples of gene TP53BP1 were expressed higher then 18-fold, 52-fold respectively. The long-range goal of this research is to provi

Type

D - Article in proceedings

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

VIIth International conference of PhD. and MSc. students "Genetics and Animal Breeding"

ISBN

978-80-7375-053-4

ISSN

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e-ISSN

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Number of pages

6

Pages from-to

78-83

Publisher name

Ediční středisko MZLU v Brně

Place of publication

Brno

Event location

Brno

Event date

May 17, 2007

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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