In vitro cultivated Dionaea muscipula as a model for investigation of cadmium detoxification by means of phytochelatins
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F07%3A00110030" target="_blank" >RIV/62156489:43210/07:00110030 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
In vitro cultivated Dionaea muscipula as a model for investigation of cadmium detoxification by means of phytochelatins
Original language description
This explantate culture was used to study the cadmium detoxification by phytochelatins and for study of influence of such stress conditions on synthesis of naphthoquinones. Dionaea muscipula explantates were cultivated for 12 weeks on unmodified MS cultivation medium as well as on modified, where all macro- and microelements in the form of sulphates were replaced by appropriate chlorides in the same concentrations. These cultivation media were enriched by cadmium in the form of cadmium chloride and cadmium acetate in the concentrations 0, 1, 5, 10, 25, 50, 100, 250, 500 and 1000 uM. The influence of cadmium acetate or cadmium sulphate on physiognomy and growth characteristics of Dionaea muscipula explantates was attentively investigated. Important changes in leaf anatomy and morphology were determined as well as growth suppression. The content of free and bound cadmium, thiol compounds and very important secondary metabolite of naphthoquinone group - plumbagine, was determined by using
Czech name
In vitro kultivovaná Dionaea muscipula jako model pro výzkum detoxikace kadmia pomocí fytochelatinů
Czech description
V této práci byly explantátové kultůry Dionaea muscipula užity ke studiu detoxikace kadmia fytochelatiny a pro studování vlivu stresových podmínek na syntézu naftochnonů. Explantáty byly kultivovány po dobu 12 týdnů v podmínkách nemodifikovaného MS kultivačního média a modifikovaného médi a, kde byly mikroelementy ve formě síranů nahrazeny chloridy o stejné koncentraci. Ke kultivačnímu mediu bylo přidáno kadmium (koncentrace 0, 1, 5, 10, 25, 50, 100, 250, 500, 1000 uM). U rostlin byly zjišťování, morfologické a růstové změny. Obash volného a váného kadmia, thiolových látek a naftochinonů byl zjištěn pomocí různých analytických metod.
Classification
Type
D - Article in proceedings
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Recent advanced in plant biotechnology
ISBN
978-80-89088-51-5
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
170-170
Publisher name
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Place of publication
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Event location
Vysoké Tatry, Slovenská republika
Event date
Jan 1, 2007
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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