Transformation of Pea to Induce Resistance to Insect Pests and Fungal Pathogens - First Partial Results
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F07%3A00117835" target="_blank" >RIV/62156489:43210/07:00117835 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Transformation of Pea to Induce Resistance to Insect Pests and Fungal Pathogens - First Partial Results
Original language description
A set of Czech pea cultivars was genetically modified with genes effecting resistance to pests and pathogens. Three constructs containing gmSPI-2 gene (Galleria mellonella silk proteinase inhibitor) were prepared for Agrobacterium-mediated transformation. Three types of regeneration system of pea derived from mature seeds (induction of trimmed seeds -- TS, cotyledonary nodes -- CN), and immature embryos (IE) were used for application of transformation protocols based on work of Švábová et al. (2005, 2007). After cocultivation, selection and regeneration steps, the potentially transformed plants were cultivated ex vitro, acclimatized and planted in a culture room to yield seed generation T1. Samples of leaf tissues were taken to test gus and bar gene activities histochemically, and then by molecular methods (PCR).
Czech name
Transformace hrachu pro indukci resitence k hmyzím škůdcům a houbovým patogenům - první dílčí výsledky
Czech description
Několik českých kultivarů hrachu bylo transformováno geny ovlivňujícími odolnost vůči škůdcům a patogenům. Tři konstrukty obsahující gmSPI-2 (Galleria mellonella silk proteinase inhibitor) byly připraveny pro transformaci hrachu pomocí Agrobakteria.
Classification
Type
D - Article in proceedings
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/1M06030" target="_blank" >1M06030: Functional genomics and proteomics for crop improvement</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Funkční genomika a proteomika ve šlechtění rostlin
ISBN
978-80-7375-090-9
ISSN
—
e-ISSN
—
Number of pages
7
Pages from-to
24-30
Publisher name
MZLU
Place of publication
Brno
Event location
—
Event date
—
Type of event by nationality
—
UT code for WoS article
—