Employing of high performance liquid chromatography with UV-VIS and electrochemical detection for determination of phenols in plants tissues

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F08%3A00134102" target="_blank" >RIV/62156489:43210/08:00134102 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Employing of high performance liquid chromatography with UV-VIS and electrochemical detection for determination of phenols in plants tissues

Original language description

The optimized parameters were as follows: column and detector temperature 42 °C, flow rate of the mobile phase 0.75 ml/min. Under these parameters well developed and symmetric signals for the target molecules were measured. The detection limits (3 S/N) for naphthoquinones and phenols were estimated below 1 uM. In addition we quantified the secondary metabolites in plant tissues. The content of naphthoquinones was determined in Nepenthes. The methanolic extracts of Nepenthes tissues (12 hours long extraction) were analysed by optimized method and high content of plumbagin with low content of lawsone was determined. Further phenolic compounds were determined in tissues of fruits (hawthorn, bilberry, shadbush, honeysuckle). The totals of flavonoid peaks express the abundance of ROS-suppressing phenolic compounds (flavonoid compounds as a main antioxidant complex) contained in the samples of less common fruit species. Based on the results the antioxidant potential at various plant species

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/EP0960006028" target="_blank" >EP0960006028: The biological quality improvement of the planting stock in fruit nurseries by means of non-convetional approaches utilization.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Vitamins 2008 - Nutrition and Diagnostics

ISBN

978-80-7318-708-8

ISSN

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e-ISSN

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Number of pages

2

Pages from-to

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Publisher name

University of Pardubice

Place of publication

Pardubice, Czech Republic

Event location

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Event date

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Type of event by nationality

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UT code for WoS article

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