Comparative analysis of ELISA, one step RT-PCR and IC-RT-PCR for the detection of Bean yellow mosaic virus in gladiolus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F09%3A00161014" target="_blank" >RIV/62156489:43210/09:00161014 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Comparative analysis of ELISA, one step RT-PCR and IC-RT-PCR for the detection of Bean yellow mosaic virus in gladiolus

Original language description

To overcome the problematic detection Bean yellow mosaic virus (BYMV) in gladiolus corm and cormlets, we tested suitability of different diagnostic methods such as ELISA, one step RT-PCR and IC-PCR. Among these methods, DAS-ELISA and one step RT-PCR wasable to detect in leaves and failed to detect in corms or cormlets whereas IC-RT-PCR method was very sensitive and able to detect virus in both gladiolus leaves and corm or cormlets.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Communications in Agricultural and Applied Biological Sciences

ISSN

1379-1176

e-ISSN

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Volume of the periodical

74

Issue of the periodical within the volume

3

Country of publishing house

BE - BELGIUM

Number of pages

7

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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