An electrochemical study of interaction of an anticancer alkaloid ellipticine with DNA

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F10%3A00166511" target="_blank" >RIV/62156489:43210/10:00166511 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
An electrochemical study of interaction of an anticancer alkaloid ellipticine with DNA
Original language description
Ellipticine (5,11-demethyl-6H-pyridol(4,3-b) carbazole) and its derivates belong to prototype compounds acting predominantly by DNA-damaging mechanisms and serving as cytotoxic agents. The aim of this work was to study the intercalation of the ellipticine into DNA. For this purpose we utilized adsorptive transfer stripping technique (AdTS) coupled with square wave voltammetry (SWV). Primarily, we investigated the influence of ellipticine on genomic DNA electrochemical signals. Decrease in redox signal of cytosine/adenine (at -1.4 V) was observed as a result of ellipticine intercalation into DNA. The decrease was proportional to increasing concentration of ellipticine. In additional experiments we investigated the changes in the reduction signal of cytosine/adenine (CA) in a genomic dsDNA, dsDNA treated with ellipticine in vitro and dsDNA isolated from UKF-NB-3 neuroblastoma cells treated with 1 mu M ellipticine. In the mentioned cases, the changes were determined.
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CG - Electrochemistry
OECD FORD branch
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)