Determination of GSH and GSSG in blood plasma of patiens by HPLC-ED

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F10%3A00184593" target="_blank" >RIV/62156489:43210/10:00184593 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Determination of GSH and GSSG in blood plasma of patiens by HPLC-ED

Original language description

Thanks to many chemicals and physical stresses generated by outside environment there still is unpredictable danger of damaging of organism by reactive oxygen species (ROS). Biological harmfulness of ROS is generally given by the subsequent oxidation ofessential cellular structures. Organism is defending against ROS by synthesis of small molecules which can neutralize radicals. The most suitable existing organic molecule widespread in whole organism which can decrease effect of ROS is glutathione (GSH). Therefore we interested in changes of GSH levels by oncological patients. We optimized fast and robust method for analysis blood plasma of patients by employment of HPLC with coulometric detection. Our results based on hunderts of analyzed samples shows significant oxidation damage by disbalance between GSH/GSSG ratio.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Biochemical Technology

ISSN

0974-2328

e-ISSN

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Volume of the periodical

2

Issue of the periodical within the volume

5

Country of publishing house

IN - INDIA

Number of pages

2

Pages from-to

9-10

UT code for WoS article

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EID of the result in the Scopus database

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