HPLC with electrochemical detection for studying of synthesis of phytochelatins in cadmium treated flax
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F10%3A00184621" target="_blank" >RIV/62156489:43210/10:00184621 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
HPLC with electrochemical detection for studying of synthesis of phytochelatins in cadmium treated flax
Original language description
If there would be free heavy metals occurring in the organism without control it initiates creation of number of dangerous species (ROS). These species are disturbing of homeostasis of organism by subsequent oxidation of essential cellular structures asnucleic acids. Animal and plant organism has both one efficient defending system which includes synthesis of glutathione (GSH). But plants are limited in case of escape from metal contaminated area. Therefore plant cell has mechanism includes synthesis of phytochelatins (PC) from GSH by phytochelatin synthase. PC's are able to immobilize heavy metal ion via number of thiol groups. We treated the plants of flax (Linum usativum) by various concentration of Cd(II) ions (50, 250, and 500mM) in our study. Subsequently we used our developed method for determination of oxidation stress as GSH/GSSG ratio and levels of PC-2,3,4 and 5. We observed that most synthesised PC under the highest concentration of Cd(II) treatment was PC-2,4 and 5.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Biochemical Technology
ISSN
0974-2328
e-ISSN
—
Volume of the periodical
2
Issue of the periodical within the volume
5
Country of publishing house
IN - INDIA
Number of pages
2
Pages from-to
11-12
UT code for WoS article
—
EID of the result in the Scopus database
—