Stanovení nového rakovinného markeru u prostatických karcinomů

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F10%3A00184801" target="_blank" >RIV/62156489:43210/10:00184801 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

Determination of novel tumor markers in prostate carcinoma

Original language description

Prostate cancer is second leading cause of death among men. In the present time, prostate specific antigen (PSA) is the only tumor marker used for carcinoma detection. This tumor marker has high level of sensitivity, but lower level of specificity, so itcan be increased either in inflammation or in prostate hyperplasia. It is desirable to find a new markers with higher level of specificity. Metallothionein (MT) and alpha-methylacyl-CoA (AMACR) racemase could be such markers. Expression of both of theseproteins is highly unregulated in prostate cancer. MT levels are elevated most likely due to disbalance of zinc metabolism in this disease, and reason of elevation of AMACR is still unknown. In our work we analyzed levels of MT, PSA and AMACR in cell lines derived from prostate carcinoma - LNCaP, PC-3 and 22RVL, which were compared to prostate cell line PNT1A derived from normal prostate epithelium. For analysis we used immunoseparation techniques, western blotting and SDS-PAGE. In addi

Czech name

Determination of novel tumor markers in prostate carcinoma

Czech description

Prostate cancer is second leading cause of death among men. In the present time, prostate specific antigen (PSA) is the only tumor marker used for carcinoma detection. This tumor marker has high level of sensitivity, but lower level of specificity, so itcan be increased either in inflammation or in prostate hyperplasia. It is desirable to find a new markers with higher level of specificity. Metallothionein (MT) and alpha-methylacyl-CoA (AMACR) racemase could be such markers. Expression of both of theseproteins is highly unregulated in prostate cancer. MT levels are elevated most likely due to disbalance of zinc metabolism in this disease, and reason of elevation of AMACR is still unknown. In our work we analyzed levels of MT, PSA and AMACR in cell lines derived from prostate carcinoma - LNCaP, PC-3 and 22RVL, which were compared to prostate cell line PNT1A derived from normal prostate epithelium. For analysis we used immunoseparation techniques, western blotting and SDS-PAGE. In addi

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GP301%2F09%2FP436" target="_blank" >GP301/09/P436: Metallothionein analysis in prostate carcinoma at DNA, RNA and protein level.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FEBS Journal

ISSN

1742-464X

e-ISSN

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Volume of the periodical

277

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

1

Pages from-to

188

UT code for WoS article

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EID of the result in the Scopus database

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