Možnosti mikroskopické detekce iontů těžkých kovů v rostlinných tkáních

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F10%3A00184942" target="_blank" >RIV/62156489:43210/10:00184942 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

Possibilities of microscopic detection of heavy metals ions in plant tissues

Original language description

Questions of environment pollution, especially by heavy metals, but also by organic pollutants, are nowadays increasingly upto-date. On this account, it is necessary to develop techniques and methods enabling detection of these pollutants. Spectroscopicmethods including fluorescence microscopy can successfully solve these questions. We are able to easily detect aromatic polyphenols and aromatic compounds in a cell, because these substances are fluorescent without addition of dyes. Ions of heavy metals(especially multivalent) as well as aromatic compounds with free -OH groups are able to bind these compounds and significantly change their ability to emit radiation after UV/VIS excitation. Our experiments were based on application of different heavy metals ions (cadmium and lead in concentrations 0, 10, 50, 100, and 500 mikroM) on hydroponically and in vitro cultivated plants of sunflower - Helianthus annuus L. Growth of experimental plants was inhibited by the presence of heavy metal

Czech name

Possibilities of microscopic detection of heavy metals ions in plant tissues

Czech description

Questions of environment pollution, especially by heavy metals, but also by organic pollutants, are nowadays increasingly upto-date. On this account, it is necessary to develop techniques and methods enabling detection of these pollutants. Spectroscopicmethods including fluorescence microscopy can successfully solve these questions. We are able to easily detect aromatic polyphenols and aromatic compounds in a cell, because these substances are fluorescent without addition of dyes. Ions of heavy metals(especially multivalent) as well as aromatic compounds with free -OH groups are able to bind these compounds and significantly change their ability to emit radiation after UV/VIS excitation. Our experiments were based on application of different heavy metals ions (cadmium and lead in concentrations 0, 10, 50, 100, and 500 mikroM) on hydroponically and in vitro cultivated plants of sunflower - Helianthus annuus L. Growth of experimental plants was inhibited by the presence of heavy metal

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Toxicology Letters

ISSN

0378-4274

e-ISSN

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Volume of the periodical

196

Issue of the periodical within the volume

6

Country of publishing house

IE - IRELAND

Number of pages

1

Pages from-to

294

UT code for WoS article

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EID of the result in the Scopus database

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