THE USE OF MICROELUTION PLATE AS A PART OF HYPHENATED EXTRACTION METHOD FOLLOWED BY RRLC-MS-MS ANALYSIS FOR THE ISOLATION AND DETERMINATION OF PHENOLIC COMPOUNDS IN SEA ALGAE
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F10%3A00234796" target="_blank" >RIV/62156489:43210/10:00234796 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2010/mendelnet_2010_fulltext.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2010/mendelnet_2010_fulltext.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
THE USE OF MICROELUTION PLATE AS A PART OF HYPHENATED EXTRACTION METHOD FOLLOWED BY RRLC-MS-MS ANALYSIS FOR THE ISOLATION AND DETERMINATION OF PHENOLIC COMPOUNDS IN SEA ALGAE
Original language description
Development and aplication of uElution plate for the extraction of phenolic compounds from sea algae and their analysis using RRLC/MS/MS is described. The extraction and identification of phenolic compounds is presented from five different sea algae samples, two brown algae (Cystoseira abies + Cystoseira abies dried with nitrogen , Undaria pinnatifida) and two red algae (Sargassum muticum, Chondrus crispus) via solid phase extraction (SPE) using Oasis uElution plate. Selected groups of benzoic acid derivatives (p-hydroxybenzoic, protocatechuic, gallic, vanillic and syringic acid), hydroxybenzaldehydes (4-hydroxybenzaldehyde and 3,4- dihydroxybenzaldehyde) and cinnamic acid derivatives (o-coumaric, p-coumaric, caffeic, ferulic, sinapic and chlorogenic acid) were investigated. Recoveries in range 96--100% were obtained, with LOQs 0.01--2.1 pg/inj and LODs 0.03--7.1 pg/inj, i.e. in the range of low um. The uSPE enabled to avoid the evaporation step and preconcentrate the analytes directly
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
GC - Plant growing, crop rotation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GP525%2F08%2FP540" target="_blank" >GP525/08/P540: On-line solid phase extraction - supercritical fluid extraction (SPE-SFE): method optimization for the extraction of isoflavones from real matrix</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2010 - Proceedings of International Ph.D. Students Conference
ISBN
978-80-7375-453-2
ISSN
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e-ISSN
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Number of pages
14
Pages from-to
999-1012
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 24, 2010
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
341786800131