Determination of lactoferrine in human saliva

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F11%3A00174187" target="_blank" >RIV/62156489:43210/11:00174187 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Determination of lactoferrine in human saliva
Original language description
Salivary is body fluid, which consists of a lot of substances as enzymes, hormones, proteins, glykoproteins, electrolytes and perform lot of tasks for body functions. Lactoferrin is a glycoprotein of about 77000 Da and into of two its domains, C- and Nterminal, can bind one iron ion to each domain. Aims of this work were to optimize FPLC separation for purification of lactoferrin from whole saliva and to determine concentration of lactoferrin in real samples of whole saliva by spectrophotometry. We analyzed of whole saliva by four healthy volunteers. After isolation of fraction which contained a purified lactoferrin this fraction was analyzed by Pyrogallol red method which was conducted by automatic spectrophotometer. Sample of whole saliva was also analyzed for determination of albumin concentrations. Then the amount of Lactoferrin was corrected on amount of albumin concentrations (ug of lactoferrin / mg of albumin) because of different physiological dilution of real sample. Obtained
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
XI. pracovní setkání fyzikálních chemiků a elektrochemiků
ISBN
978-80-7375-514-0
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
327-331
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
MENDELU
Event date
Jan 1, 2011
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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