Separation of lactoferrin from human saliva using monolithic disc

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A00213595" target="_blank" >RIV/62156489:43210/13:00213595 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26620/13:PU126741

Result on the web

<a href="http://dx.doi.org/10.1007/s10337-013-2459-x" target="_blank" >http://dx.doi.org/10.1007/s10337-013-2459-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10337-013-2459-x" target="_blank" >10.1007/s10337-013-2459-x</a>

Result language

angličtina

Original language name

Separation of lactoferrin from human saliva using monolithic disc

Original language description

In this study, we optimized method for the isolation and detection of lactoferrin from human saliva using 3 mm short monolithic disc. We optimized the conditions for separation as flow rate 4 mL min-1 and ionic strength of effluent as 2 uM NaCl. We estimated limit of detection of whole method, which was hyphenated to the Bradford's assay, down to 100 ng mL-1. The purity of the isolated fractions was verified by sodium dodecyl sulphatepolyacrylamide gel electrophoresis and recovery of isolation was foundto be 51 % using minimally processed sample of saliva. Further, we tested the optimized method on group of healthy volunteers (n = 7). We were able to distinguish between the healthy subjects and subject suffering from celiac disease, which reported atleast 2.59 higher level of lactoferrin in comparison to healthy ones. The results were correlated with standard enzyme-linked immunosorbent assay (ELISA) kit with obtained correlation coefficient R2 = 0.8446. Analysis of lactoferrin in sa

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chromatographia

ISSN

0009-5893

e-ISSN

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Volume of the periodical

76

Issue of the periodical within the volume

11-12

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

611-619

UT code for WoS article

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EID of the result in the Scopus database

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