It provides Colors are anthocyanins in samples of wheat grain during maturation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F11%3A00179423" target="_blank" >RIV/62156489:43210/11:00179423 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

Stanovni antokyanu ve vzorcich barevne psenice v prubehu zrani zrna

Original language description

Extraction of anthocyanins from pigmented wheat grains and their analysis using HPLC/MS is described. Grain from six wheat lines differing in pigmentation was used in this study. Wheat grains were harvested every five days beginning 15th day post anthesis, ending once the grains reached physiologic maturity (40th day post anthesis), for a total six sampling dates. Anthocyanins were extracted with 10 ml acidified methanol (methanol and 1, 0 N HCl, 85:15 v/v) and shaking on Ika Ultra- Turrax Tube Drive, for 2x29 min. The filtered extract were evaporated at 50 °C to dryness and redissolved in acidifield methanol. Individual anthocyanins were separated and quantified by high-performance liquid chromatography (HPLC). Anthocyanin concentration increased rapidly during grain development and then decreased before maturity. Cyanidin-3- glucoside was the predominant anthocyanin in purple wheat. Delphinidin-3-glucoside is the principal one in blue wheat. Anthocyanins are considered to be physiolo

Czech name

Stanovni antokyanu ve vzorcich barevne psenice v prubehu zrani zrna

Czech description

Extraction of anthocyanins from pigmented wheat grains and their analysis using HPLC/MS is described. Grain from six wheat lines differing in pigmentation was used in this study. Wheat grains were harvested every five days beginning 15th day post anthesis, ending once the grains reached physiologic maturity (40th day post anthesis), for a total six sampling dates. Anthocyanins were extracted with 10 ml acidified methanol (methanol and 1, 0 N HCl, 85:15 v/v) and shaking on Ika Ultra- Turrax Tube Drive, for 2x29 min. The filtered extract were evaporated at 50 °C to dryness and redissolved in acidifield methanol. Individual anthocyanins were separated and quantified by high-performance liquid chromatography (HPLC). Anthocyanin concentration increased rapidly during grain development and then decreased before maturity. Cyanidin-3- glucoside was the predominant anthocyanin in purple wheat. Delphinidin-3-glucoside is the principal one in blue wheat. Anthocyanins are considered to be physiolo

Type

D - Article in proceedings

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

18. Nove poznatky z genetiky a slachtenia polnohospodarskych rastlin

ISBN

978-80-89417-29-2

ISSN

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e-ISSN

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Number of pages

8

Pages from-to

1003-1010

Publisher name

Centrum vyskumu rastlinnej vyroby Piestany

Place of publication

Piestany, Slovensko

Event location

Piestany, Slovensko

Event date

Jan 1, 2011

Type of event by nationality

CST - Celostátní akce

UT code for WoS article

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