The effect of Staphylococcus aureus bacteria to proportion of gamma delta T-lymphocytes from bovine mammary gland
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A43908860" target="_blank" >RIV/62156489:43210/13:43908860 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2013/mnet_2013_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2013/mnet_2013_full.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The effect of Staphylococcus aureus bacteria to proportion of gamma delta T-lymphocytes from bovine mammary gland
Original language description
The aim of this thesis was study of lymphocytes, especially subpopulation gamma delta T-lymphocytes. The experiment was conducted on five clinically healthy heifers. Heifers were crossbred Holstein and Czech Pied. Their mammary glands were experimentallyinfected by bacteria Staphylococcus aureus. Bacterial suspension was applied in amout of 5 ml and the concentration was 800 CFU/ml. Application of inoculum was designed after control lavage with phosphate buffered saline (PBS). Samples of cell populations were obtain by lavage of the mammary gland in 4 intervals (24, 48, 72 and 168 hrs) after initiation of experirimental infection. Percentage of lymphocytes and subpopulation gamma delta T-lymphocytes were detected by flow cytometry. Stimulation of mammary gland by S. aureus led to percetnage increase of lymhocytes from 9.62% detected in 24 hrs to 27.36% in 168 hrs after aplication of inoculum. Percentage increase of gamma delta T-lymphocytes was detected too. 24 hrs after stimulation b
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
GJ - Diseases and animal vermin, veterinary medicine
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2013: Proceedings of International PhD Students Conference
ISBN
978-80-7375-908-7
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
788-792
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 20, 2013
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000366464800138