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The effect of Staphylococcus aureus bacteria to proportion of gamma delta T-lymphocytes from bovine mammary gland

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A43908860" target="_blank" >RIV/62156489:43210/13:43908860 - isvavai.cz</a>

  • Result on the web

    <a href="https://mnet.mendelu.cz/mendelnet2013/mnet_2013_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2013/mnet_2013_full.pdf</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    The effect of Staphylococcus aureus bacteria to proportion of gamma delta T-lymphocytes from bovine mammary gland

  • Original language description

    The aim of this thesis was study of lymphocytes, especially subpopulation gamma delta T-lymphocytes. The experiment was conducted on five clinically healthy heifers. Heifers were crossbred Holstein and Czech Pied. Their mammary glands were experimentallyinfected by bacteria Staphylococcus aureus. Bacterial suspension was applied in amout of 5 ml and the concentration was 800 CFU/ml. Application of inoculum was designed after control lavage with phosphate buffered saline (PBS). Samples of cell populations were obtain by lavage of the mammary gland in 4 intervals (24, 48, 72 and 168 hrs) after initiation of experirimental infection. Percentage of lymphocytes and subpopulation gamma delta T-lymphocytes were detected by flow cytometry. Stimulation of mammary gland by S. aureus led to percetnage increase of lymhocytes from 9.62% detected in 24 hrs to 27.36% in 168 hrs after aplication of inoculum. Percentage increase of gamma delta T-lymphocytes was detected too. 24 hrs after stimulation b

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    GJ - Diseases and animal vermin, veterinary medicine

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2013

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    MendelNet 2013: Proceedings of International PhD Students Conference

  • ISBN

    978-80-7375-908-7

  • ISSN

  • e-ISSN

  • Number of pages

    5

  • Pages from-to

    788-792

  • Publisher name

    Mendelova univerzita v Brně

  • Place of publication

    Brno

  • Event location

    Brno

  • Event date

    Nov 20, 2013

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article

    000366464800138