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Fluorescence resonance energy transfer between green fluorescent protein and doxorubicin enabled by DNA nanotechnology

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F14%3A43908666" target="_blank" >RIV/62156489:43210/14:43908666 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216305:26620/14:PU126725

  • Result on the web

    <a href="http://dx.doi.org/10.1002/elps.201400166" target="_blank" >http://dx.doi.org/10.1002/elps.201400166</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/elps.201400166" target="_blank" >10.1002/elps.201400166</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Fluorescence resonance energy transfer between green fluorescent protein and doxorubicin enabled by DNA nanotechnology

  • Original language description

    DNA nanotechnology is a rapidly growing research area, where DNA may be used for wide range of applications such as construction of nanodevices serving for large scale of diverse purposes. Likewise a panel of various purified fluorescent proteins is investigated for their ability to emit their typical fluorescence spectra under influence of particular excitation. Hence these proteins may form ideal donor molecules for assembly of fluorescence resonance emission transfer (FRET) constructions. To extend the application possibilities of fluorescent proteins, while using DNA nanotechnology, we developed nanoconstruction comprising green fluorescent protein (GFP) bound onto surface of surface active nanomaghemite and functionalized with gold nanoparticles.We took advantage of natural affinity between gold and thiol moieties, which were modified to bind DNA fragment. Finally we enclosed doxorubicin into fullerene cages. Doxorubicin intercalated in DNA fragment bound on the particles and thu

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Electrophoresis

  • ISSN

    0173-0835

  • e-ISSN

  • Volume of the periodical

    35

  • Issue of the periodical within the volume

    23

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    12

  • Pages from-to

    3290-3301

  • UT code for WoS article

    000345972900002

  • EID of the result in the Scopus database