Interactions of fluorescent dye SYBR Green I with natural and 7-deazaguanine-modified DNA studied by fluorescence and electrochemical methods

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F16%3A00456297" target="_blank" >RIV/68081707:_____/16:00456297 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14740/16:00090268

Result on the web

<a href="http://dx.doi.org/10.1007/s00706-015-1578-5" target="_blank" >http://dx.doi.org/10.1007/s00706-015-1578-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-015-1578-5" target="_blank" >10.1007/s00706-015-1578-5</a>

Result language

angličtina

Original language name

Interactions of fluorescent dye SYBR Green I with natural and 7-deazaguanine-modified DNA studied by fluorescence and electrochemical methods

Original language description

YBR Green I (SG) is a fluorescent dye applied in various techniques of DNA analysis, including fluorescent staining of electrophoretic gels, quantitative polymerase chain reaction, etc. SG binds selectively to double-stranded DNA via intercalation and minor groove interactions, resulting in a considerable enhancement of fluorescence of the dye. Modification of DNA by partial or full replacement of natural purine nucleobase guanine (G) with its synthetic analog 7-deazaguanine (G*) or its derivatives wasshown to cause the SG fluorescence quenching. In this paper, we present a comparative study of interactions of SG with natural DNA fragments and with DNA fragments modified with G* by means of fluorescence and electrochemical methods. Competition betweenunmodified (forming strongly fluorescent complex with SG) and fully G*-modified (not contributing significantly to overall fluorescence signal) DNA fragments for the dye was studied via changes in the fluorescence intensity.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Monatshefte fur Chemie

ISSN

0026-9247

e-ISSN

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Volume of the periodical

147

Issue of the periodical within the volume

1

Country of publishing house

AT - AUSTRIA

Number of pages

8

Pages from-to

13-20

UT code for WoS article

000367521400003

EID of the result in the Scopus database

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