Electrochemical Analysis of Sybr Green I and its Interaction with DNA

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F15%3A00506701" target="_blank" >RIV/68081707:_____/15:00506701 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Electrochemical Analysis of Sybr Green I and its Interaction with DNA

Original language description

There are used many methods such real-time PCR and electrophoresis in molecular biology and biochemistry which are based on utilization of a fluorescent dye Sybr Green I (SG) for selective detection of double stranded (ds) DNA and its quantification. SG is a planar molecule (see Fig. 1) that intercalates into the DNA double helix and simultaneously can bind into minor groove of dsDNA. In our work we focused on electrochemical behavior of SG on a pyrolytic graphite electrode (PGE) using square-wave voltammetry (SWV). The voltammetric method was used in measurements of SG interactions with DNA at the PGE.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project

<a href="/en/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Center of novel approaches to bioanalysis and molecular diagnostics</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

XXXV. Moderní elektrochemické metody. Sborník přednášek mezinárodní odborné konference

ISBN

978-80-905221-3-8

ISSN

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e-ISSN

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Number of pages

3

Pages from-to

"Č. 2015 (2015)"

Publisher name

Srsenová Lenka - Best Servis

Place of publication

Ústí nad Labem

Event location

Jetřichovice

Event date

May 18, 2015

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000358792900007