Oxidation of Sanguinarine and Its Dihydro-Derivative at a Pyrolytic Graphite Electrode Using Ex Situ Voltammetry. Study of the Interactions of the Alkaloids with DNA

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F11%3A10222937" target="_blank" >RIV/61989592:15110/11:10222937 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/11:00370858 RIV/61989592:15310/11:10222937

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Oxidation of Sanguinarine and Its Dihydro-Derivative at a Pyrolytic Graphite Electrode Using Ex Situ Voltammetry. Study of the Interactions of the Alkaloids with DNA

Original language description

This study describes oxidation of sanguinarine (SG) and its metabolite dihydrosanguinarine (DHSG) on the surface of a basal-plane pyrolytic graphite electrode (PGE). Since both alkaloids strongly adsorb onto the surface of pyrolytic graphite, measurements were performed using ex situ voltammetric methods, adsorptive transfer (AdT) cyclic voltammetry (CV) and square-wave voltammetry (SWV). The electrochemical results and optimized AdT SWV were subsequently applied for study of the interactions of SG andDHSG with DNA in vitro. Electrochemical study of the interactions was supported and complemented with measurements using gel electrophoresis and fluorescence spectroscopy. The results suggest that SG intercalates into the double-stranded structure of scDNA while increased binding affinity was observed for quaternary cation (SG+). DHSG which, unlike SG+, does not possess a strictly planar molecular structure, did not show intercalative DNA binding in any of the three methods applied.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CG - Electrochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electroanalysis

ISSN

1040-0397

e-ISSN

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Volume of the periodical

23

Issue of the periodical within the volume

7

Country of publishing house

DE - GERMANY

Number of pages

10

Pages from-to

1671-1680

UT code for WoS article

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EID of the result in the Scopus database

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