Fluorescence-tagged metallothionein with CdTe quantum dots analyzed by the chip-CE technique

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43906766" target="_blank" >RIV/62156489:43210/15:43906766 - isvavai.cz</a>
Result on the web
<a href="http://link.springer.com/content/pdf/10.1007%2Fs11051-015-3226-8.pdf" target="_blank" >http://link.springer.com/content/pdf/10.1007%2Fs11051-015-3226-8.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11051-015-3226-8" target="_blank" >10.1007/s11051-015-3226-8</a>

Result language
angličtina
Original language name
Fluorescence-tagged metallothionein with CdTe quantum dots analyzed by the chip-CE technique
Original language description
Quantum dots (QDs) are fluorescence nanoparticles (NPs) with unique optic properties which allow their use as probes in chemical, biological, immunological, and molecular imaging. QDs linked with target ligands such as peptides or small molecules can beused as tumor biomarkers. These particles are a promising tool for selective, fast, and sensitive tagging and imaging in medicine. In this study, an attempt was made to use QDs as a marker for human metallothionein (MT) isoforms 1 and 2. Four kinds of CdTe QDs of different sizes bioconjugated with MT were analyzed using the chip-CE technique. Based on the results, it can be concluded that MT is willing to interact with QDs, and the chip-CE technique enables the observation of their complexes. It was also observed that changes ranging roughly 6-7 kDa, a value corresponding to the MT monomer, depend on the hydrodynamic diameters of QDs; also, the MT sample without cadmium interacted stronger with QDs than MT saturated with cadmium. Result
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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