Rapid identification of bacteria by biobarcode assay

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43906879" target="_blank" >RIV/62156489:43210/15:43906879 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2015/mnet_2015_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2015/mnet_2015_full.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Rapid identification of bacteria by biobarcode assay
Original language description
Presence of bacteria with antibiotic resistance is becoming a very large problem throughout the world. Methicillin-resistant Staphylococcus aureus (MRSA) is a dangerous pathogen resistant to ?-lactam antibiotics with biofilm-formation ability. Because ofan increasing resistance of bacterial species to ATBs, it is necessary to develop new methods for rapid identification of bacteria. Biobarcode assay provides a rapid detection of the antigen presence in the sample. Detection is based on the antibody-antigen interaction. The antibodies were bound to magnetic and non-magnetic particles. Next, immunoglobulin G (IgG) was bounded to magnetic particles. The non-magnetic particles were bound with anti-plasminogen antibody that is a specific antigen for MRSA as well as 20 bp oligonucleotides for detection. The first step involved determination of binding capacity of antibodies for different bacteria by ELISA. The IgG were able to bind 4.6 .104 +- 8% CFU . ml-1 of MRSA, Escherichia coli and Pro
Czech name
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Czech description
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Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2015: Proceedings of International PhD Students Conference
ISBN
978-80-7509-363-9
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
448-452
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 11, 2015
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000366466100084

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