Particle-based immunochemical separation of methicillin resistant Staphylococcus aureus with indirect electrochemical detection of labeling oligonucleotides
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43910481" target="_blank" >RIV/62156489:43210/16:43910481 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/16:PU119672
Result on the web
<a href="http://dx.doi.org/10.1039/c6ay01296e" target="_blank" >http://dx.doi.org/10.1039/c6ay01296e</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/c6ay01296e" target="_blank" >10.1039/c6ay01296e</a>
Alternative languages
Result language
angličtina
Original language name
Particle-based immunochemical separation of methicillin resistant Staphylococcus aureus with indirect electrochemical detection of labeling oligonucleotides
Original language description
Early detection of antibiotic-resistant bacteria causing inflammation in patients is a key for an appropriate and timely treatment. Classical microbiological cultivation methods are time-consuming and therefore it is necessary to discover a quick and effective method. The method used in this study is based on a sandwich immunoreaction of bacteria with IgG immobilized on streptavidin-coated polystyrene superparamagnetic particles and MRSA-specific antibody immobilized on gold non-magnetic nanoparticles labeled with oligonucleotides, which were detected by square wave voltammetry in combination with the adsorptive transfer technique. The assay is suitable for selective detection of methicillin-resistant Staphylococcus aureus in samples even at a concentration of 2 x 10(4) CFU mL(-1). The method can be flexibly modified for any other bacteria, depending on the antibodies used.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Methods
ISSN
1759-9660
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
25
Country of publishing house
GB - UNITED KINGDOM
Number of pages
6
Pages from-to
5123-5128
UT code for WoS article
000378941500020
EID of the result in the Scopus database
2-s2.0-84976417386