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Prion protein and its interactions with metal ions (Cu2 , Zn2 , and Cd2 ) and metallothionein 3

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43907826" target="_blank" >RIV/62156489:43210/15:43907826 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216305:26620/15:PU136027

  • Result on the web

    <a href="http://dx.doi.org/10.5599/admet.3.3.191" target="_blank" >http://dx.doi.org/10.5599/admet.3.3.191</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.5599/admet.3.3.191" target="_blank" >10.5599/admet.3.3.191</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Prion protein and its interactions with metal ions (Cu2 , Zn2 , and Cd2 ) and metallothionein 3

  • Original language description

    The effects of heavy metals (Zn2+, Cu2+, and/or Cd2+) on Escherichia coli expressing either prion (hPrPC ) or metallothionein 3 (MT-3) brain proteins capable of binding these metals were investigated. The expression of hPrPC or MT-3 in E.coli was confirmed using western-blot and dot-blot methods. After analyzing growth curves, we found that bacteria expressing prion protein better tolerated the presence of Zn2+ in comparison with wild-type bacteria and bacteria expressing MT-3. The addition of Cd2+ and Cu2+ was well tolerated by bacteria expressing MT-3, whereas the bacteria expressing prion protein displayed slower growth when compared to the wild-type. We subsequently determined total content of the MT in bacteria using differential pulsed voltammetry (DPV), and depending on the treatment of the individual metals. MT expression in MT3 transformed cells as well as in control E.coli cells increased at the lowest metal concentration (25 μM), followed by a decrease at higher metal concentrations (50, 75, and 150 μM). The highest increase by Cd2+ were observed. MT expression pattern in hPrPC transformed cells was different. After application of Cu2+ an increase in MT expression continued also at higher metal concentrations; and after application of Cd2+ and Zn2+ no decrease in MT expression at higher metal concentrations was observed

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30104 - Pharmacology and pharmacy

Result continuities

  • Project

    <a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    ADMET &amp; DMPK

  • ISSN

    1848-7718

  • e-ISSN

  • Volume of the periodical

    3

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    HR - CROATIA

  • Number of pages

    9

  • Pages from-to

    287-295

  • UT code for WoS article

    000447335900013

  • EID of the result in the Scopus database

    2-s2.0-85047052672