Generation of Arabidopsis Lines with Altered Cytokinin Level Expressing GFP-Fused Cytoskeletal Proteins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43910205" target="_blank" >RIV/62156489:43210/16:43910205 - isvavai.cz</a>

Result on the web

<a href="https://mnet.mendelu.cz/mendelnet2016/mnet_2016_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2016/mnet_2016_full.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Generation of Arabidopsis Lines with Altered Cytokinin Level Expressing GFP-Fused Cytoskeletal Proteins

Original language description

Cell division, expansion and differentiation require a sophisticated spatial arrangement of the cytoskeleton. The ever increasing progress in fluorescent microscope techniques has allowed to visualize rearrangements and dynamics of actin and microtubule arrays in various cell types and tissues in plants. Processes controlling proper cytoskeleton organisation and its response to various stimuli, mediated by actin and microtubule binding proteins as well as plant hormones, are being an area of active investigation. As the current research of cytoskeleton regulation focused mainly on hormone auxin, the involvement of cytokinin is still unclear. In this study, we describe generation of transgenic lines of Arabidopsis thaliana comprising inducible system for manipulation of endogenous cytokinin level and simultaneously GFP-labelled actin or microtubule proteins. These lines serve as a tool for the study of cytokinin mode of action in the course of cell/organ development. Based on our results, it is obvious that cytokinins play a considerable role in modulation of plant cytoskeleton. However, further research is necessary to elucidate precise cytokinin signalling pathways and cross-talk with other hormones, which participate in such complex processes of cell morphology.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

MendelNet 2016: Proceedings of International PhD Students Conference

ISBN

978-80-7509-443-8

ISSN

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e-ISSN

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Number of pages

6

Pages from-to

757-762

Publisher name

Mendelova univerzita v Brně

Place of publication

Brno

Event location

Brno

Event date

Nov 9, 2016

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

000392968500135