Determination of S-Adenosylmethionine and S-Adenosylhomocysteine in Two Prostatic Adenocarcinoma Cell Lines
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43910232" target="_blank" >RIV/62156489:43210/16:43910232 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/16:PU122568
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2016/mnet_2016_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2016/mnet_2016_full.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Determination of S-Adenosylmethionine and S-Adenosylhomocysteine in Two Prostatic Adenocarcinoma Cell Lines
Original language description
Studies of processes leading to cancer tumour progression are one of the major foci of current cancer research. Therefore, it is important to shade the light on the metabolic pathways involved in cancerogenesis. In the present study we have chosen as a model two prostatic adenocarcinoma cell lines (PC-3 and LNCaP) for the comparison of the metabolites involved in tumour growth. Applying the HPLC-MS method, we determined and compared the concentration levels of S-(5'-adenosyl)-L-homocysteine (SAH) and S-(5'-adenosyl)-L-methionine (SAM), that are involved in the tumour growth processes. SAM/SAH ratio, a methylation index describing the aggressiveness of tumour cells, was determined here. The methylation index was 0.4 and 1.2 for PC-3 and LNCaP cells, respectively, pointing out that the PC-3 cells are more aggressive than LNCaP cells. This finding is of great importance for our following studies.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA16-18917S" target="_blank" >GA16-18917S: The study of sarcosine metabolism and its participation in prostate cancer development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2016: Proceedings of International PhD Students Conference
ISBN
978-80-7509-443-8
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
972-977
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 9, 2016
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000392968500174