Cryo-electron microscopy study of the genome release of the dicistrovirus Israeli acute bee paralysis virus

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43910281" target="_blank" >RIV/62156489:43210/17:43910281 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/17:00096372
Result on the web
<a href="http://dx.doi.org/10.1128/JVI.02060-16" target="_blank" >http://dx.doi.org/10.1128/JVI.02060-16</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/JVI.02060-16" target="_blank" >10.1128/JVI.02060-16</a>

Result language
angličtina
Original language name
Cryo-electron microscopy study of the genome release of the dicistrovirus Israeli acute bee paralysis virus
Original language description
Viruses from the family Dicistroviridae can cause substantial economic damage by infecting agriculturally important insects. Israeli acute bee paralysis virus (IAPV) causes honeybee colony collapse disorder in the United States. High-resolution molecular details of the genome delivery mechanism of dicistroviruses are unknown. Here we present a cryo-EM analysis of IAPV virions induced to release their genomes in vitro. We determined structures of full IAPV virions primed to release their genomes to a resolution of 3.3 Å and of empty capsids to a resolution of 3.9 Å. We show that IAPV does not form expanded A particles before the genome release, as is the case in the related enteroviruses from the family Picornaviridae. The structural changes observed in the empty IAPV particles include the detachment of the VP4 minor capsid proteins from the inner face of the capsid and partial loss of the structure of the N-terminal arms of the VP2 capsid proteins. Unlike in many picornaviruses, the empty particles of IAPV are not expanded, relative to the native virions, and do not contain pores in their capsids that might serve as channels for the genome release. Therefore, re-arrangement of a unique region of the capsid is probably required for IAPV genome release.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10607 - Virology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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