Cryo-EM study of slow bee paralysis virus at low pH reveals iflavirus genome release mechanism

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43910710" target="_blank" >RIV/62156489:43210/17:43910710 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14740/17:00096214

Result on the web

<a href="http://dx.doi.org/10.1073/pnas.1616562114" target="_blank" >http://dx.doi.org/10.1073/pnas.1616562114</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1073/pnas.1616562114" target="_blank" >10.1073/pnas.1616562114</a>

Result language

angličtina

Original language name

Cryo-EM study of slow bee paralysis virus at low pH reveals iflavirus genome release mechanism

Original language description

Viruses from the family Iflaviridae are insect pathogens. Many of them, including slow bee paralysis virus (SBPV), cause lethal diseases in honeybees and bumblebees, resulting in agricultural losses. Iflaviruses have nonenveloped icosahedral virions containing single-stranded RNA genomes. However, their genome release mechanism is unknown. Here, we show that low pH promotes SBPV genome release, indicating that the virus may use endosomes to enter host cells. We used cryo-EM to study a heterogeneous population of SBPV virions at pH 5.5. We determined the structures of SBPV particles before and after genome release to resolutions of 3.3 and 3.4 angstrom, respectively. The capsids of SBPV virions in low pH are not expanded. Thus, SBPV does not appear to form &quot;altered&quot; particles with pores in their capsids before genome release, as is the case in many related picornaviruses. The egress of the genome from SBPV virions is associated with a loss of interpentamer contacts mediated by N-terminal arms of VP2 capsid proteins, which result in the expansion of the capsid. Pores that are 7 angstrom an diameter form around icosahedral threefold symmetry axes. We speculate that they serve as channels for the genome release. Our findings provide an atomic-level characterization of the genome release mechanism of iflaviruses.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10607 - Virology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Proceedings of the National Academy of Sciences of the United States of America

ISSN

0027-8424

e-ISSN

—

Volume of the periodical

114

Issue of the periodical within the volume

3

Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

598-603

UT code for WoS article

000392095800054

EID of the result in the Scopus database

2-s2.0-85009723978