Using CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43911543" target="_blank" >RIV/62156489:43210/17:43911543 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/17:PU123082
Result on the web
<a href="https://doi.org/10.2147/IJN.S121840" target="_blank" >https://doi.org/10.2147/IJN.S121840</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2147/IJN.S121840" target="_blank" >10.2147/IJN.S121840</a>
Alternative languages
Result language
angličtina
Original language name
Using CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA
Original language description
CdTe/ZnSe core/shell quantum dot (QD), one of the strongest and most highly luminescent nanoparticles, was directly synthesized in an aqueous medium to study its individual interactions with important nucleobases (adenine, guanine, cytosine, and thymine) in detail. The results obtained from the optical analyses indicated that the interactions of the QDs with different nucleobases were different, which reflected in different fluorescent emission maxima and intensities. The difference in the interaction was found due to the different chemical behavior and different sizes of the formed nanoconjugates. An electrochemical study also confirmed that the purines and pyrimidines show different interactions with the core/shell QDs. Based on these phenomena, a novel QD-based method is developed to detect the presence of the DNA, damage to DNA, and mutation. The QDs were successfully applied very easily to detect any change in the sequence (mutation) of DNA. The QDs also showed their ability to detect DNAs directly from the extracts of human cancer (PC3) and normal (PNT1A) cells (detection limit of 500 pM of DNA), which indicates the possibilities to use this easy assay technique to confirm the presence of living organisms in extreme environments.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Nanomedicine
ISSN
1176-9114
e-ISSN
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Volume of the periodical
12
Issue of the periodical within the volume
14 February
Country of publishing house
NZ - NEW ZEALAND
Number of pages
15
Pages from-to
1277-1291
UT code for WoS article
000394047900003
EID of the result in the Scopus database
2-s2.0-85013187207