DNA interaction with platinum-based cytostatics revealed by DNA sequencing

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43912469" target="_blank" >RIV/62156489:43210/17:43912469 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/17:00100210 RIV/00216208:11130/17:10369484 RIV/00216208:11310/17:10369484 RIV/00216305:26620/17:PU125955 RIV/00064203:_____/17:10369484
Result on the web
<a href="https://doi.org/10.1016/j.ab.2017.09.018" target="_blank" >https://doi.org/10.1016/j.ab.2017.09.018</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ab.2017.09.018" target="_blank" >10.1016/j.ab.2017.09.018</a>

Result language
angličtina
Original language name
DNA interaction with platinum-based cytostatics revealed by DNA sequencing
Original language description
The main mechanism of action of platinum-based cytostatic drugs - cisplatin, oxaliplatin and carboplatin - is the formation of DNA cross-links, which restricts the transcription due to the disability of DNA to enter the active site of the polymerase. The polymerase chain reaction (PCR) was employed as a simplified model of the amplification process in the cell nucleus. PCR with fluorescently labelled dideoxynucleotides commonly employed for DNA sequencing was used to monitor the effect of platinum-based cytostatics on DNA in terms of decrease in labeling efficiency dependent on a presence of the DNA-drug cross-link. It was found that significantly different amounts of the drugs - cisplatin (0.21 μg/mL), oxaliplatin (5.23 μg/mL), and carboplatin (71.11 μg/mL) - were required to cause the same quenching effect (50%) on the fluorescent labelling of 50 μg/mL of DNA. Moreover, it was found that even though the amounts of the drugs was applied to the reaction mixture differing by several orders of magnitude, the amount of incorporated platinum, quantified by inductively coupled plasma mass spectrometry, was in all cases at the level of tenths of μg per 5 μg of DNA.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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