New option for decreasing of concentration limit of detection in electrophoresis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43912627" target="_blank" >RIV/62156489:43210/17:43912627 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
New option for decreasing of concentration limit of detection in electrophoresis
Original language description
A hyphenated method consisting of transient electrokinetic dosing on-line coupled to isotachophoretic analysis was developed for the pre-concentration, pre-separation and analytical determination of a model substance - anionic herbicide glyphosate (gly) - in aqueous samples containing very low concentration of the analyte of interest. Various parameters were investigated in the framework of an optimisation study; the aim was to reach minimal concentration limit of detection decreasing in minimum time. The developed method consists of 2 phases. In the first one, a sample with addition of convenient buffer (dosing electrolyte) is electrokinetically dosed to the isotachophoretic column with proper leading electrolyte. During the dosing time, a moving-boundary electrophoresis zone of accumulated sample is created in the column and it is slowly moving through column. The accumulation of zone is proportional to the time and driving current and to the composition of dosing electrolyte. After some time of accumulation of the zone, dosing electrolyte is replaced with terminating one. Now, the regular isotachophoretic separation and analysis starts. The electrolyte composition and the dosing time were thoroughly optimized and 14 fold of accumulation was reached in 25 minutes in comparison to classical isotachophoretic analysis. The method is simple and applicable to all commercial isotachophoretic analysers.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2017: Proceedings of International PhD Students Conference
ISBN
978-80-7509-529-9
ISSN
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e-ISSN
neuvedeno
Number of pages
5
Pages from-to
884-888
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 8, 2017
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000440194500158