Improving the detection limit in capillary isotachophoresis using asymmetric neutralisation reaction boundary
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F22%3A43920395" target="_blank" >RIV/62156489:43210/22:43920395 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1002/elps.202000398" target="_blank" >https://doi.org/10.1002/elps.202000398</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.202000398" target="_blank" >10.1002/elps.202000398</a>
Alternative languages
Result language
angličtina
Original language name
Improving the detection limit in capillary isotachophoresis using asymmetric neutralisation reaction boundary
Original language description
An on-line method involving transient electrokinetic dosing and ITP with neutralisation reaction boundary (NRB) and/or carrier ampholyte-free isoelectric focusing (CAF IEF) was developed for the pre-concentration, pre-separation, and analytical determination of glyphosate in aqueous samples containing low concentrations of the analyte of interest. Various parameters were investigated in the framework of an optimisation study with the aim of achieving the maximum concentration limit of detection (cLOD) decrease in minimum time. The proposed method used CAF IEF and/or ITP with NRB. The sample was dosed to the column on the stationary reaction boundary (CAF IEF) and/or moving reaction boundary (ITP with NRB), whereat a sharp pH step exists. Here, charge reversal was due to the ampholytes and/or acid accumulation occurred because of charge loss. Similarly, the accumulated sample was mobilised with TE and analysed using classical ITP in the second analytical column. Glyphosate (GLY), the analyte of interest, was chosen as a model substance for ITP with NRB and pre-concentration as well as focusing pre-concentration and CAF IEF using the asymmetric purpose-built NRB. On one side of the asymmetric boundary was the zone of acidic pH; while the opposite side comprised a neutral/basic non-conductive zone of the ampholyte-in this case, GLY. Such an arrangement enables the utilisation of a lower pH on the acidic side, which allows the focusing of strongly acidic ampholytes and the accumulation of weak acids. The electrolyte composition and the dosing time were optimised, and a 14-fold accumulation was achieved in 25 min compared to that by classical ITP and a 180-fold accumulation was achieved through CAF IEF and pre-concentration with a glyphosate sample. Both methods are simple and can be conducted using all commercial ITP systems.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
1522-2683
Volume of the periodical
43
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
417-424
UT code for WoS article
000709191700001
EID of the result in the Scopus database
2-s2.0-85117376664