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Multifunctional pipeteing platform for molecular biology and biochemistry

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43912640" target="_blank" >RIV/62156489:43210/17:43912640 - isvavai.cz</a>

  • Result on the web

    <a href="https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Multifunctional pipeteing platform for molecular biology and biochemistry

  • Original language description

    The classical hand pipetting approach can be partially substituted in several cases by sophisticated devices that are taking over the necessity of sample manipulation. The developed pipetting method can facilitate routine laboratory work, but the created method has to meet several criteria including - minimizing the possibility of contamination. In this work is briefly summarized four years experiences with the liquid handling system BioNex Nanodrop II. This device is able to pipette (using eight pipetting tips) small volumes in the range of 0.100 µl up to 500 µl into the three common microplate formats - MTP 96, MTP 384 and MTP 1536. The possibility of sample (bacteria, bacterial plasmids and DNA templates for PCR) cross-contamination was estimated in three experiments. The cross-contamination was not presented or it was detected at very low level. The main advantages - speed and accuracy - of the automatic pipetting are described in the last experiment where the enzyme activity was measured.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

  • OECD FORD branch

    10609 - Biochemical research methods

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    MendelNet 2017: Proceedings of International PhD Students Conference

  • ISBN

    978-80-7509-529-9

  • ISSN

  • e-ISSN

    neuvedeno

  • Number of pages

    6

  • Pages from-to

    943-948

  • Publisher name

    Mendelova univerzita v Brně

  • Place of publication

    Brno

  • Event location

    Brno

  • Event date

    Nov 8, 2017

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article

    000440194500169