Multifunctional pipeteing platform for molecular biology and biochemistry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43912640" target="_blank" >RIV/62156489:43210/17:43912640 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf</a>
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Multifunctional pipeteing platform for molecular biology and biochemistry
Original language description
The classical hand pipetting approach can be partially substituted in several cases by sophisticated devices that are taking over the necessity of sample manipulation. The developed pipetting method can facilitate routine laboratory work, but the created method has to meet several criteria including - minimizing the possibility of contamination. In this work is briefly summarized four years experiences with the liquid handling system BioNex Nanodrop II. This device is able to pipette (using eight pipetting tips) small volumes in the range of 0.100 µl up to 500 µl into the three common microplate formats - MTP 96, MTP 384 and MTP 1536. The possibility of sample (bacteria, bacterial plasmids and DNA templates for PCR) cross-contamination was estimated in three experiments. The cross-contamination was not presented or it was detected at very low level. The main advantages - speed and accuracy - of the automatic pipetting are described in the last experiment where the enzyme activity was measured.
Czech name
—
Czech description
—
Classification
Type
D - Article in proceedings
CEP classification
—
OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2017: Proceedings of International PhD Students Conference
ISBN
978-80-7509-529-9
ISSN
—
e-ISSN
neuvedeno
Number of pages
6
Pages from-to
943-948
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 8, 2017
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000440194500169