Upconversion nanoparticle bioconjugates characterized by capillary electrophoresis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43913917" target="_blank" >RIV/62156489:43210/18:43913917 - isvavai.cz</a>
Alternative codes found
RIV/68081715:_____/18:00493528 RIV/00216305:26620/18:PU129007
Result on the web
<a href="https://doi.org/10.1002/elps.201700483" target="_blank" >https://doi.org/10.1002/elps.201700483</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201700483" target="_blank" >10.1002/elps.201700483</a>
Alternative languages
Result language
angličtina
Original language name
Upconversion nanoparticle bioconjugates characterized by capillary electrophoresis
Original language description
Upconversion nanoparticles (UCNPs) are an emerging class of optical materials with high potential in bioimaging due to practically no background signal and high penetration depth. Their excellent optical properties and easy surface functionalization make them perfect for conjugation with targeting ligands. In this work, capillary electrophoretic (CE) method with laser-induced fluorescence detection was used to investigate the behavior of carboxyl-silica-coated UCNPs. Folic acid, targeting folate receptor overexpressed by wide variety of cancer cells, was used for illustrative purposes and assessed by CE under optimized conditions. Peptide-mediated bioconjugation of antibodies to UCNPs was also investigated. Despite the numerous advantages of CE, this is the first time that CE was employed for characterization of UCNPs and their bioconjugates. The separation conditions were optimized including the background electrolyte concentration and pH. The optimized electrolyte was 20 mM borate buffer with pH 8.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
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Volume of the periodical
39
Issue of the periodical within the volume
17
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
2246-2252
UT code for WoS article
000443558300009
EID of the result in the Scopus database
2-s2.0-85050587178