Molecular detection of fungi in paprika, chili powder and black pepper

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43914084" target="_blank" >RIV/62156489:43210/18:43914084 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.11118/actaun201866040927" target="_blank" >https://doi.org/10.11118/actaun201866040927</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.11118/actaun201866040927" target="_blank" >10.11118/actaun201866040927</a>

Result language

angličtina

Original language name

Molecular detection of fungi in paprika, chili powder and black pepper

Original language description

Paprika powder, chili powder, and black pepper are among the most frequently used spices in the world. The Internal Transcribed Spacer (ITS) regions were identified by high sequence similarity with the ITS regions of many microscopic fungi, especially representatives of the phylum Ascomycota, from 18 different spice samples that were examined. However, supplied quality certificates indicated that 10 of the 18 samples had no contamination present and were safe for human consumption. The various genera of fungi that were identified from the spices are considered to be a food safety concern as they are able to produce mycotoxins. Qualitative detection was supplemented by positive detection of viable fungal DNA using qPCR for the genera Aspergillus/Penicillium in two paprika powder and black pepper samples. These results concurred with the control analysis using axenic cultures. The described methods can be used for routine testing of spices to provide safe spices and other products to consumers.

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

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OECD FORD branch

40401 - Agricultural biotechnology and food biotechnology

Project

<a href="/en/project/TG02010074" target="_blank" >TG02010074: MENDELU Proof-of-Concept Project</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis

ISSN

1211-8516

e-ISSN

—

Volume of the periodical

66

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

11

Pages from-to

927-937

UT code for WoS article

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EID of the result in the Scopus database

2-s2.0-85054594921