Utilization of antibody-nanoparticle conjugates as a tool for immunochemistry with ICP-MS detection

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43914719" target="_blank" >RIV/62156489:43210/18:43914719 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/19:PU131645 RIV/00216224:14310/18:00113396
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Utilization of antibody-nanoparticle conjugates as a tool for immunochemistry with ICP-MS detection
Original language description
Immunoanalytical techniques are key methods of application in clinical diagnostics, genomics, proteomics and other biochemical and molecular biology disciplines. Most often, they are based on the ability of labeled antibodies to bind specific antigens. It is possible to use a large variety of nanomaterials that are designed, synthesized and adapted to allow highly sensitive detection of advanced immunoassays. Detection can be a highly efficient analytical method of laser ablation followed by inductively coupled plasma mass spectrometry (LA-ICP-MS), which allows the detection of elemental tags suitably conjugated to antibodies. The aim of this work was to conjugate model anti-mouse antibody on a surface of 10nm and 60nm gold nanoparticles and choose the better one for conjugation experimentally by using dot-blot immunobinding assay followed by LA-ICP-MS. It has been experimentally proven that 10nm gold nanoparticles are more suitable for conjugation with antibodies because of lower non-specific sorption on a membrane.
Czech name
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Czech description
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Project
<a href="/en/project/GA17-12774S" target="_blank" >GA17-12774S: Metal-nanopartcials based multilabeling for bioimaging</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2018: Proceedings of International PhD Students Conference
ISBN
978-80-7509-597-8
ISSN
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e-ISSN
neuvedeno
Number of pages
5
Pages from-to
548-552
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 7, 2018
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000462205300104

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