LA-ICP-MS as a sensitive method for detection of nanoparticle-antibody conjugates in immunochemistry analysis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F19%3APU134937" target="_blank" >RIV/00216305:26620/19:PU134937 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/19:43917147 RIV/00216224:14310/19:00114693
Result on the web
<a href="https://mendelnet.cz/artkey/mnt-201901-0131_LA-ICP-MS-as-a-sensitive-method-for-detection-of-nanoparticle-antibody-conjugates-in-immunochemistry-analysis.php?back=/magno/mnt/2019/mn1.php?secid=4" target="_blank" >https://mendelnet.cz/artkey/mnt-201901-0131_LA-ICP-MS-as-a-sensitive-method-for-detection-of-nanoparticle-antibody-conjugates-in-immunochemistry-analysis.php?back=/magno/mnt/2019/mn1.php?secid=4</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
LA-ICP-MS as a sensitive method for detection of nanoparticle-antibody conjugates in immunochemistry analysis
Original language description
Laser ablation followed by inductively coupled plasma mass spectrometry is an analytical method suitable for low concentration measurement of elements in a sample. This can be used not only for detection of elemental distribution within a sample but also as a detection means for immunoanalytical techniques using nanoparticle-antibody conjugates as recognition elements. The aim of this work was to prepare a conjugate of 10nm gold nanoparticles with anti-p53 antibody, to verify the antigen binding ability, and to use this construct for identification of p53 in a real sample of cell lysate. It has been experimentally verified that it is possible to use nanoparticle-modified antibodies for immunochemical analysis coupled to LA-ICP-MS detection. However, the nonspecific sorption of nanoparticles on the sample surface needs to be minimized by optimization of the blocking step.
Czech name
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Czech description
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Project
<a href="/en/project/GA17-12774S" target="_blank" >GA17-12774S: Metal-nanopartcials based multilabeling for bioimaging</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2019 Proceedings of 26th International PhD Students Conference
ISBN
978-80-7509-688-3
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
682-686
Publisher name
Mendel University in Brno
Place of publication
Neuveden
Event location
Brno
Event date
Nov 6, 2019
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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