One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F19%3A43915941" target="_blank" >RIV/62156489:43210/19:43915941 - isvavai.cz</a>

Alternative codes found

RIV/00159816:_____/19:00071271 RIV/00216224:14110/19:00107803 RIV/00216305:26620/19:PU133552

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0039914019307362/pdfft?md5=960be23496441c780b05a49dff954f0c&pid=1-s2.0-S0039914019307362-main.pdf" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0039914019307362/pdfft?md5=960be23496441c780b05a49dff954f0c&pid=1-s2.0-S0039914019307362-main.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2019.07.006" target="_blank" >10.1016/j.talanta.2019.07.006</a>

Result language

angličtina

Original language name

One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity

Original language description

Due to the close relationship between carcinogenesis and human papillomavirus (HPV), and since they are transmitted via huge number of asymptomatic carriers, the detection of HPV is really needed to reduce the risk of developing cancer. According to the best of our knowledge, our study provides the very first method for one-step detection of viral infection and if it has initiated the subsequent cancer proliferation. The proposed novel nanosystem consists of magnetic glass particles (MGPs), which were attached with DNA probe on their surface to hybridize with target DNAs. The MGP-probe-DNA hybrid was finally conjugated with CdTe/ZnSe core/shell quantum dots (QDs). The proposed detection system is based on a novel mechanism in which the MGPs separate out the target DNAs from different biological samples using external magnetic field for better and clear detection and the QDs give different fluorescent maxima for different target DNAs due to their ability to interact differently with different nucleotides. Firstly, the method was optimized using HPV genes cloned into synthetic plasmids. Then it was applied directly on the samples from normal and cancerous cells. After that, the real hospital samples of head and neck squamous cell carcinoma (HNSCC) with or without the infection of HPV were also analyzed. Our novel nano-system is proved successful in detecting and distinguishing between the patients suffering by HPV infection with or without subsequent cancer having detection limit estimated as 1.0 x 109 (GEq/mL). The proposed methodology is faster and cost-effective, which can be applied at the clinical level to help the doctors to decide the strategy of medication that may save the life of the patients with an early treatment.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Talanta

ISSN

0039-9140

e-ISSN

—

Volume of the periodical

205

Issue of the periodical within the volume

December

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

"UNSP 120111"

UT code for WoS article

000485856500047

EID of the result in the Scopus database

2-s2.0-85068523227