Ion-Exchange Based Immobilization of Chromogenic Reagents on Microfluidic Paper Analytical Devices

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F19%3A43916144" target="_blank" >RIV/62156489:43210/19:43916144 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26620/19:PU132996

Result on the web

<a href="https://doi.org/10.1021/acs.analchem.9b01288" target="_blank" >https://doi.org/10.1021/acs.analchem.9b01288</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.analchem.9b01288" target="_blank" >10.1021/acs.analchem.9b01288</a>

Result language

angličtina

Original language name

Ion-Exchange Based Immobilization of Chromogenic Reagents on Microfluidic Paper Analytical Devices

Original language description

Distance-based detection methods, as used in development of microfluidic paper analytical devices (mu PADs), rely on the dynamic formation of a colored band along the length of the paper microfluidic channels. The color change is driven by the reaction of chromogenic reagents (typically water-insoluble) that are bound to the paper, thus not subject to being washed away by the sample flow along the detection channel. Here, we introduce the use of an anion-exchange filter paper (as a replacement for standard, unmodified filter paper) for distance-based detection in mu PADs, in order to immobilize the water-soluble anionic reagents upon the paper detection channels based on ion-exchange interactions of the oppositely charged paper (protonated tertiary amine groups) and the anionic groups of the reagents. The ion-exchange (IE) paper was initially characterized and its properties were compared with standard cellulose paper. The IE paper was shown to be capable of strong retention of anionic reagents exhibiting acidic functional groups (carboxylic, sulfonic), which become deprotonated and negatively charged when in contact with the IE paper. The effect of the ionic strength (10-250 mM Cl-) and pH (1-13) on the immobilization of the investigated reagents were also determined. The IE-mu PADs were then modified with anionic chromogenic reagents and applied to distance-based determination of total calcium (LOD = 0.03 mM) and total acidity (LOD = 2.5 mM) content in serum and wine samples, respectively. The detailed mechanisms of the developed assays on the IE paper are also discussed. We propose that IE-mu PADs represent a useful new addition to the distance-based detection toolbox and considerably enhance the applicability of such a detection method.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical Chemistry

ISSN

0003-2700

e-ISSN

—

Volume of the periodical

91

Issue of the periodical within the volume

14

Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

8756-8761

UT code for WoS article

000476567700006

EID of the result in the Scopus database

2-s2.0-85069949189