The Effect of Muramyl Dipeptide and Lipopolysaccharide on Expression of CD14 And CD44 by Bovine Mammary Gland Neutrophils In Vitro

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F19%3A43916590" target="_blank" >RIV/62156489:43210/19:43916590 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.15414/jmbfs.2019.9.special.467-472" target="_blank" >http://dx.doi.org/10.15414/jmbfs.2019.9.special.467-472</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.15414/jmbfs.2019.9.special.467-472" target="_blank" >10.15414/jmbfs.2019.9.special.467-472</a>

Result language

angličtina

Original language name

The Effect of Muramyl Dipeptide and Lipopolysaccharide on Expression of CD14 And CD44 by Bovine Mammary Gland Neutrophils In Vitro

Original language description

Previous studies indicate neutrophils&apos; expression of CD14 and CD44 is associated with stage of inflammatory response. This study, undertaken in vitro investigates whether bacterial components affect neutrophils&apos; CD14 and CD44 expression and lifespan. Neutrophils, obtained through lavage of mammary glands, were incubated with three different concentrations of muramyl dipeptide (MDP) and lipopolysaccharide (LPS). Percentages of apoptotic neutrophils, and of CD14+ and CD44+ neutrophils, were detected after 30, 60, 120 and 300 minutes of incubation. A high concentration of MDP produced a significantly higher percentage of CD14+ after 30 and 120 minutes, compared with LPS and the control, for which percentages remained constant. CD44+ increased at low concentrations of MDP and LPS after 120 minutes, and at 60 minutes for intermediate concentrations. High concentrations resulted in significant increase, this after only 30 minutes for MDP. Analysis of variance indicated incubation time followed by bacterial component, as most important in expression of CD14 and CD44.

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

—

OECD FORD branch

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Microbiology, Biotechnology and Food Science

ISSN

1338-5178

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

Special Issue

Country of publishing house

SK - SLOVAKIA

Number of pages

6

Pages from-to

467-472

UT code for WoS article

000500544500024

EID of the result in the Scopus database

2-s2.0-85083702586