Muramyl dipeptide can influence mammary gland lymphocytes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F19%3A43917116" target="_blank" >RIV/62156489:43210/19:43917116 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2019/mnet_2019_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2019/mnet_2019_full.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Muramyl dipeptide can influence mammary gland lymphocytes
Original language description
Mammary gland is important organ for all calf. The mammary gland of the bovine is divided lengthwise into left and right halves and is further divided into anterior and posterior quarters. Inflammation of the mammary gland is one of the most serious production diseases of dairy cattle. Lukocytes play an important role during mammary gland inflammation. During inflammation, leukocytes move from the blood to the mammary gland. Programmed leukocyte cell death plays an important role in the inflammatory response of the mammary gland. The study was implemented on eight healthy heifers in age 16 to 18 months. Each mammary gland in each quarter of the udder was injected with 20 ml phosphate buffered saline (PBS) with 500 μg of muramyl dipeptide synthetic derivate. The lymphocytes obtained by lavages of mammary glands were analysed by flow cytometry and subsequently by software WinMDI 2.8. Proportion of apoptotic lymphocytes was enumerated by staining with Annexin-V and propidium iodide. The cytokine concentrations were determined using sandwich ELISA. Apoptosis of lymphocytes and concentration of TNF-α were peaked 48 hours following stimulation of the mammary glands by muramyl dipeptide. We have found high correlation in initial stage of mastitis between apoptosis of lymphocytes and TNF-α production especially in 48 hours after experimentally induced inflammation.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
40201 - Animal and dairy science; (Animal biotechnology to be 4.4)
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2019: Proceedings of International PhD Students Conference
ISBN
978-80-7509-688-3
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
485-489
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 6, 2019
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000576735500087