FRET as a powerful tool to study protein dimerization

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F19%3A43917132" target="_blank" >RIV/62156489:43210/19:43917132 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2019/mnet_2019_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2019/mnet_2019_full.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
FRET as a powerful tool to study protein dimerization
Original language description
Herein, Förster resonance energy transfer (FRET) was used to investigate the oligomerization of mammalian metallothionein (MT) isoform MT-1. A FRET system was developed based on a highly fluorescent ZnCd quantum dot (QD) and cyanine 3 (Cy3) as a powerful tool to probe small distance changes between acceptor and donor fluorophores in nanometer range. In this study, the water-soluble 450-nm emitting ZnCd QDs as donor and 570-nm Cy3 as acceptor were covalently conjugated with MT-1. MetallothioneinMT1 forms dimers (as well as higher oligomers) upon storing under aerobic conditions. These dimers/oligomers were investigated using capillary electrophoresis (CE) coupled with fluorescence detection.
Czech name
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Czech description
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Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2019: Proceedings of International PhD Students Conference
ISBN
978-80-7509-688-3
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
591-595
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 6, 2019
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000576735500106

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