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Using QD-FRET based method to investigate protein-protein interactions

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43919821" target="_blank" >RIV/62156489:43210/21:43919821 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216305:26620/21:PU141192

  • Result on the web

    <a href="https://doi.org/10.37904/nanocon.2020.3750" target="_blank" >https://doi.org/10.37904/nanocon.2020.3750</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.37904/nanocon.2020.3750" target="_blank" >10.37904/nanocon.2020.3750</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Using QD-FRET based method to investigate protein-protein interactions

  • Original language description

    Förster resonance energy transfer (FRET) is an energy transfer process between a pair of light-sensitive molecules, where the donor fluorophore (initially in its electronic excited state) transfers energy to an acceptor chromophore. In this study, FRET was used to investigate the dimerization of metallothionein isoform MT-1A. The FRET system was developed based on a fluorescent quantum dots (QDs) and cyanine dye 3.5 (Cy3.5) as a versatile tool to probe small distance changes between acceptor and donor fluorophores in nanometer range. Herein, the water-soluble 450-nm emitting QDs as the donor and 590-nm Cy3.5 as the acceptor were covalently conjugated with MT-1 according to the protocol. Previous studies suggest that MTs may form oligomers under certain conditions. Therefore, further studies of this phenomenon, which has been studied here using capillary electrophoresis (CE) coupled with fluorescence detection.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

  • OECD FORD branch

    10406 - Analytical chemistry

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    NANOCON 2020: Conference Proceedings

  • ISBN

    978-80-87294-98-7

  • ISSN

    2694-930X

  • e-ISSN

    2694-930X

  • Number of pages

    5

  • Pages from-to

    390-394

  • Publisher name

    Tanger Ltd.

  • Place of publication

    Ostrava

  • Event location

    Brno

  • Event date

    Oct 21, 2020

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article

    000664505500066