Complex cytotoxicity mechanism of bundles formed from self-organised 1-D anodic TiO2 nanotubes layers

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43917433" target="_blank" >RIV/62156489:43210/20:43917433 - isvavai.cz</a>

Alternative codes found

RIV/00216275:25310/20:39916344 RIV/00216305:26620/20:PU136535

Result on the web

<a href="https://doi.org/10.1016/j.jhazmat.2020.122054" target="_blank" >https://doi.org/10.1016/j.jhazmat.2020.122054</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jhazmat.2020.122054" target="_blank" >10.1016/j.jhazmat.2020.122054</a>

Result language

angličtina

Original language name

Complex cytotoxicity mechanism of bundles formed from self-organised 1-D anodic TiO2 nanotubes layers

Original language description

The present study reports on a comprehensive investigation of mechanisms of in vitro cytotoxicity of high aspect ratio (HAR) bundles formed from anodic TiO2 nanotube (TNT) layers. Comparative cytotoxicity studies were performed using two types of HAR TNTs (diameter of TILDE OPERATOR+D91110 nm), differing in initial thickness of the nanotubular layer (TILDE OPERATOR+D9135 μm for TNTs-1 vs. TILDE OPERATOR+D9110 μm for TNTs-2). Using two types of epithelial cell lines (MDA-MB-231, HEK-293), it was found that nanotoxicity is highly cell-type dependent and plausibly associates with higher membrane fluidity and decreased rigidity of cancer cells enabling penetration of TNTs to the cell membrane towards disruption of membrane integrity and reorganization of cytoskeletal network. Upon penetration, TNTs dysregulated redox homeostasis followed by DNA fragmentation and apoptotic/necrotic cell death. Both TNTs exhibited haemolytic activity and rapidly activated polarization of RAW 264.7 macrophages. Throughout the whole study, TNTs-2 possessing a lower aspect ratio manifested significantly higher cytotoxic effects. Taken together, this is the first report comprehensively investigating the mechanisms underlying the nanotoxicity of bundles formed from self-organised 1-D anodic TNT layers. Except for description of nanotoxicity of industrially-interesting nanomaterials, the delineation of the nanotoxicity paradigm in cancer cells could serve as solid basis for future efforts in rational engineering of TNTs towards selective anticancer nanomedicine.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30108 - Toxicology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Hazardous Materials

ISSN

0304-3894

e-ISSN

—

Volume of the periodical

388

Issue of the periodical within the volume

15 April

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

12

Pages from-to

122054

UT code for WoS article

000524462500109

EID of the result in the Scopus database

2-s2.0-85077754730