Bundles formed from self-organized 1-D-anodic TiO2 nanotubes layers: assessment of nanotoxicity using human epithelial cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43918633" target="_blank" >RIV/62156489:43210/20:43918633 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26620/20:PU137972

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Bundles formed from self-organized 1-D-anodic TiO2 nanotubes layers: assessment of nanotoxicity using human epithelial cells

Original language description

In recent years, society has witnessed unparalleled growth of research of various nanomaterials that has resulted in a broad spectrum of their innovative applications in various fields of medicine and industry. Current statistics suggest that there are more than 1000 products available world-wide that take advantage of nanotechnology. Titanium dioxide (TiO2) nanoparticle is an important product for nanotechnology because of its high stability, anticor-rosion and photocatalysis. It is frequently used in the cosmetics, pharmaceutical, paint, and paper industries. TiO2 has been previously classified as biologically inert; however, our recent study revealed that small (diameter of ~6 nm) TiO2 nanoparticles exhibit inherent cytotoxicity for mammalian cells. It has been shown that high aspect ratio (HAR) materials have a dramatic impact when in contact with living organism as compared to their 0-D forms. The present study reports on a comprehensive investigation of mechanisms of in vitro cytotoxicity of high aspect ratio (HAR) bundles formed from TiO2 nanotube (TNT) layers prepared via electrochemical anodization of Ti foils. Comparative cytotoxicity stud-ies were performed using two types of HAR TNTs with similar inner diameter of ~110 nm, differing in initial thickness of the nanotubular layer (~35pm for TNTs-1 vs. ~10pm for TNTs-2). Using two types of epithelial cell lines (MDA-MB-231, HEK-293), it was found that nanotoxicity is highly cell-type dependent and plausibly associates with higher membrane fluidity and decreased rigidity of cancer (MDA-MB-231) cells enabling simpler penetration of TNTs to the cell membrane towards disruption of membrane integrity and reorganization of F-actin cytoskeletal network. Upon penetration, TNTs dysregulated redox homeostasis followed by DNA frag-mentation and apoptotic/necrotic cell death. In addition, both TNTs exhibited haemolytic activity and rapidly activated polarization of RAW 264.7 macrophages. The clarification of the fundamental TNTs cytotoxicity para-digm in cancer cells provide additional opportunities for a future engineering (tuning of size or surface chemistry) of highly sophisticated rational cancer nanomedicines exploiting differences in cell responses to TNTs.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/EF16_025%2F0007314" target="_blank" >EF16_025/0007314: Multidisciplinary research to increase application potential of nanomaterials in agricultural practice</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů