Immunoassay of Glomalin by Quartz Crystal Microbalance Biosensor Containing Iron Oxide Nanoparticles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43918260" target="_blank" >RIV/62156489:43210/20:43918260 - isvavai.cz</a>
Alternative codes found
RIV/60162694:G44__/20:00556179
Result on the web
<a href="https://doi.org/10.1155/2020/8844151" target="_blank" >https://doi.org/10.1155/2020/8844151</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1155/2020/8844151" target="_blank" >10.1155/2020/8844151</a>
Alternative languages
Result language
angličtina
Original language name
Immunoassay of Glomalin by Quartz Crystal Microbalance Biosensor Containing Iron Oxide Nanoparticles
Original language description
Glomalin is a soil protein resembling heat shock protein (HSP) 60 and exerting high affinity to metals, causing retention of water in the environment and improving mechanical stability of soil. Currently, glomalin is determined in the soil or other samples by combination of autoclaving extraction and total protein determination typically by the Bradford method. In this paper, a piezoelectric biosensor was prepared to determine glomalin in a label-free measurement. The biosensor contained antibodies immobilized on quartz crystal microbalance (QCM), and the recognition layer was stabilized by iron oxide nanoparticles. The assay was tested on real soil samples and compared with the standard Bradford assay. Limit of detection of the assay was equal to 2.4 µg/g for a soil extract with a volume of 50 µl. The assay takes approximately half of an hour and was fully correlated to the Bradford assay. The biosensor had significant advantages than the other methods: it worked in a label-free mode and was fully applicable for practical samples.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Analytical Chemistry
ISSN
1687-8760
e-ISSN
—
Volume of the periodical
20
Issue of the periodical within the volume
1 September
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
8844151
UT code for WoS article
000571862300003
EID of the result in the Scopus database
2-s2.0-85091998692