QCM immunosensor for the determination of Staphylococcus aureus antigen

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F20%3A00555659" target="_blank" >RIV/60162694:G44__/20:00555659 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007%2Fs11696-019-00889-5" target="_blank" >https://link.springer.com/article/10.1007%2Fs11696-019-00889-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11696-019-00889-5" target="_blank" >10.1007/s11696-019-00889-5</a>

Result language

angličtina

Original language name

QCM immunosensor for the determination of Staphylococcus aureus antigen

Original language description

Staphylococcus aureus is a causative agent of infectious diseases and a producer of a group of highly toxic proteins called Staphylococcal enterotoxins. Currently, the presence of S. aureus in a sample can be determined by a wide spectrum of genetic, immunochemical and microbiological techniques. Despite availability of standard methods and devices, they are not fully suitable for a simple, label-free mode of use by an unskilled worker. In this paper, a biosensor based on quartz crystal microbalance (QCM) sensor is performed as a tool for a simple and reliable determination of S. aureus. The biosensor contained an antibody specific to S. aureus immobilized through protein A on 10 MHz QCM sensors and the interdigitated layer was stabilized by iron nanoparticles. The assay was performed as label free and sample was applied without any pretreatment. Limit of detection 145 mu g/ml (5.18 x 10(8) CFU/ml) was achieved. When considered standard size of a sample 50 mu l, the limit of detection was equal to 7.25 mu g (2.59 x -10(7) CFU). The biosensors kept more than 90% sensitivity to the analyte after storage in laboratory conditions for 90 days. Good correlation to standard enzyme-linked immunosorbent assay was also proved. The biosensor appears as a simple but reliable tool for S. aureus assay and the QCM platform would provide further applications in microbial analyses.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10400 - Chemical sciences

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemical Papers

ISSN

2585-7290

e-ISSN

1336-9075

Volume of the periodical

74

Issue of the periodical within the volume

2

Country of publishing house

CH - SWITZERLAND

Number of pages

8

Pages from-to

451-458

UT code for WoS article

000514312600007

EID of the result in the Scopus database

2-s2.0-85069875607